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Product Info Summary
| SKU: | LS003125 |
|---|---|
| Size: | 100 µg |
| Expression Host: | HEK293T |
| Tag: | C-Myc/DDK |
| Description: | Transient overexpression lysate of major histocompatibility complex, class II, DR beta 3 (HLA-DRB3) |
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Product info
Product Name
HLA-DRB3 (NM_022555) Human Over-expression Lysate
SKU/Catalog Number
LS003125
Size
100 µg
Description
Transient overexpression lysate of major histocompatibility complex, class II, DR beta 3 (HLA-DRB3)
Storage & Handling
Cite This Product
HLA-DRB3 (NM_022555) Human Over-expression Lysate (Boster Biological Technology, Pleasanton CA, USA, Catalog # LS003125)
Tag
C-Myc/DDK
Detection Antibodies
Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (M30971)
Components
1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer
1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer
1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT)
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with Transfection Reagent and 5ug ORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit. Cell lysates were aliquoted and stored at -20°C before shipping.
Assay Dilution & Images
Validation Images & Assay Conditions
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HLA-DRB3 Human Over-expression Lysates NM_022555.
Western blot validation of overexpression lysate (LS003125) using anti-DDK antibody. Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with HLA (Myc-DDK-tagged) Human Tagged ORF Clone using transfection reagent.
Protein Target Info & Infographic
Gene/Protein Information for HLA-DRB3 (Source: Uniprot.org, NCBI)
Gene Name
HLA-DRB3
Full Name
HLA class II histocompatibility antigen, DR beta 3 chain
Weight
29.962kDa
Superfamily
MHC class II family
Alternative Names
DR7; HLA class II histocompatibility antigen, DR beta 3 chain; HLA class II histocompatibility antigen, DRB1-7 beta chain; HLA-DR3B; HLA-DR52; HLA-DRB1; human leucocyte antigen DRB3; major histocompatibility complex, class II, DR beta 3; MGC117330; MHC class II antigen DR beta 3 chain; MHC class II antigen DRB3; MHC class II HLA-DR beta 3 chain HLA-DRB3 DRB3, HLA-DPB1, HLA-DR1B, HLA-DR3B*, HLA-DRB3 major histocompatibility complex, class II, DR beta 3 major histocompatibility complex, class II, DR beta 3|HLA DRB3|HLA class II histocompatibility antigen, DR beta 3 chain|MHC class II DR beta chain|MHC class II HLA-DR beta 3 chain|MHC class II antigen DR beta 3 chain|MHC class II antigen DRB3|MHC class II protein|human leucocyte antigen DRB3
*If product is indicated to react with multiple species, protein information is based on the gene entry specified above in "Species".For more info on HLA-DRB3, check out the HLA-DRB3 Infographic
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In this infographic, you will see the following information for HLA-DRB3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For HLA-DRB3 (NM_022555) Human Over-expression Lysate (LS003125)
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